Mouse SAMHD1 Recombinant Protein (N-His) (MP056012)

Name: Mouse SAMHD1 Recombinant Protein (N-His)
Brand: abinScience
SKU: MP056012
Price: 211 USD
Availability: InStock

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Número de catálogo	MP056012
Descripción	Recombinant Mouse SAMHD1 Protein, N-His (MP056012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q60710
Longitud de proteína	Asp145-Phe658
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Mus musculus (Mouse)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	62.08 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, EC:3.1.5.-, Interferon-gamma-inducible protein Mg11, Mg21, SAM domain and HD domain-containing protein 1, Samhd1, dNTPase, mSAMHD1
Fondo	Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 is a ~75 kDa protein. Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks. Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools. Phosphorylation at Thr-634 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks. 1. Rehwinkel, J. et al. (2013) The EMBO journal 32, 2454-62. PMID: 23872947 2. Behrendt, R. et al. (2013) Cell reports 4, 689-96. PMID: 23972988 3. Wittmann, S. et al. (2015) Retrovirology 12, 103. PMID: 26667483 4. Buzovetsky, O. et al. (2018) Nature communications 9, 411. PMID: 29379009 5. Deutschmann, J. et al. (2019) Nature microbiology 4, 2273-2284. PMID: 31548683 6. Thientosapol, ES. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 4921-4926. PMID: 29669924
Nota	For research use only

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Datasheet

Mouse SAMHD1 Recombinant Protein (N-His).pdf

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