Mouse SAMHD1 Recombinant Protein (N-His) (MP056012)

Name: Mouse SAMHD1 Recombinant Protein (N-His)
Brand: abinScience
SKU: MP056012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	MP056012
Description	Recombinant Mouse SAMHD1 Protein, N-His (MP056012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q60710
Longueur de protéine	Asp145-Phe658
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Mus musculus (Mouse)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	62.08 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, EC:3.1.5.-, Interferon-gamma-inducible protein Mg11, Mg21, SAM domain and HD domain-containing protein 1, Samhd1, dNTPase, mSAMHD1
Contexte	Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 is a ~75 kDa protein. Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks. Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools. Phosphorylation at Thr-634 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks. 1. Rehwinkel, J. et al. (2013) The EMBO journal 32, 2454-62. PMID: 23872947 2. Behrendt, R. et al. (2013) Cell reports 4, 689-96. PMID: 23972988 3. Wittmann, S. et al. (2015) Retrovirology 12, 103. PMID: 26667483 4. Buzovetsky, O. et al. (2018) Nature communications 9, 411. PMID: 29379009 5. Deutschmann, J. et al. (2019) Nature microbiology 4, 2273-2284. PMID: 31548683 6. Thientosapol, ES. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 4921-4926. PMID: 29669924
Note	For research use only

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Mouse SAMHD1 Recombinant Protein (N-His).pdf

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