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Обзор
Каталожный номерMB024012
Описание
Recombinant Mouse UCP1 Protein, N-GST & C-His (MB024012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Система экспрессииE. coli
Номер доступаP12242
Длина белкаPro33-Lys73&Thr134-Thr178&Val233-Ala266
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыMus musculus (Mouse)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияMitochondrial brown fat uncoupling protein 1, Slc25a7, Solute carrier family 25 member 7, Thermogenin, UCP 1, Ucp, Ucp1
Фон

Mitochondrial brown fat uncoupling protein 1 is a ~33 kDa protein. Mitochondrial transporter that functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Regulates the production of reactive oxygen species/ROS by mitochondria.

1. Fedorenko, A. et al. (2012) Cell 151, 400-13. PMID: 23063128
2. Feldmann, HM. et al. (2009) Cell metabolism 9, 203-9. PMID: 19187776
3. Chouchani, ET. et al. (2016) Nature 532, 112-6. PMID: 27027295
4. Enerbäck, S. et al. (1997) Nature 387, 90-4. PMID: 9139827
5. Dlasková, A. et al. (2010) Biochimica et biophysica acta 1797, 1470-6. PMID: 20416274
6. Oelkrug, R. et al. (2010) The Journal of biological chemistry 285, 21961-8. PMID: 20466728
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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