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| カタログ番号 | MY404012 |
|---|---|
| 説明 |
Recombinant Mouse UNG Protein, N-His (MY404012) expressed in E. coli, spanning Ser60-Leu306. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P97931 |
| タンパク質長 | Ser60-Leu306 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mus musculus (Mouse) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 30.14 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DGU, EC:3.2.2.27, UDG, UNG, UNG1, UNG15, Uracil-DNA glycosylase |
| 背景 | Uracil-DNA glycosylase is a ~33 kDa protein. Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine. Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication. 1. Torseth, K. et al. (2012) DNA repair 11, 559-69. PMID: 22521144 2. Nilsen, H. et al. (1997) Nucleic acids research 25, 750-5. PMID: 9016624 3. Rada, C. et al. (2002) Current biology : CB 12, 1748-55. PMID: 12401169 4. Maul, RW. et al. (2011) Nature immunology 12, 70-6. PMID: 21151102 5. Schrader, CE. et al. (2005) The Journal of experimental medicine 202, 561-8. PMID: 16103411 |
| 注意事項 | For research use only |
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