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| カタログ番号 | MB820012 |
|---|---|
| 説明 |
Recombinant Mouse ZFP36 Protein, N-His (MB820012) expressed in E. coli, spanning Thr91-Pro160. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P22893 |
| タンパク質長 | Thr91-Pro160 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mus musculus (Mouse) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 10.42 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | mRNA decay activator protein ZFP36, Growth factor-inducible nuclear protein NUP475, TPA-induced sequence 11, Tristetraprolin, Zinc finger protein 36, Zfp-36, Zfp36, Nup475, Tis11, Tis11a, Ttp |
| 背景 | mRNA decay activator protein ZFP36 is a ~33 kDa protein. Zinc-finger RNA-binding protein that destabilizes numerous cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as an 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1, and hence promotes ARE-mediated mRNA deadenylation. Also functions by recruiting components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs. Self-regulates by destabilizing its own mRNA. 1. Lai, WS. et al. (1999) Molecular and cellular biology 19, 4311-23. PMID: 10330172 2. Carballo, E. et al. (2000) Blood 95, 1891-9. PMID: 10706852 3. Stoecklin, G. et al. (2000) Molecular and cellular biology 20, 3753-63. PMID: 10805719 4. Stoecklin, G. et al. (2004) The EMBO journal 23, 1313-24. PMID: 15014438 5. Tchen, CR. et al. (2004) The Journal of biological chemistry 279, 32393-400. PMID: 15187092 6. Ogilvie, RL. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 174, 953-61. PMID: 15634918 7. Lai, WS. et al. (2006) Molecular and cellular biology 26, 9196-208. PMID: 17030620 8. Horner, TJ. et al. (2009) Molecular and cellular biology 29, 1999-2010. PMID: 19188452 9. Marchese, FP. et al. (2010) The Journal of biological chemistry 285, 27590-600. PMID: 20595389 10. Clement, SL. et al. (2011) Molecular and cellular biology 31, 256-66. PMID: 21078877 |
| 注意事項 | For research use only |
SDS-PAGE for Recombinant Mouse ZFP36 Protein, N-His
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