MRV-3 Sigma3/S4 Recombinant Protein (N-His) (VK094022)

Name: MRV-3 Sigma3/S4 Recombinant Protein (N-His)
Brand: abinScience
SKU: VK094022
Price: 211 USD
Availability: InStock

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Número de catálogo	VK094022
Descripción	Recombinant MRV-3 Sigma3/S4 Protein, N-His (VK094022) expressed in E. coli, spanning Met1-Gly365. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P03527
Longitud de proteína	Met1-Gly365
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Mammalian orthoreovirus 3 (MRV3)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	43.28 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	S4
Fondo	Outer capsid protein sigma-3 is a ~41 kDa protein. Outer layer capsid protein that stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding. During virus assembly, the inner layer proteins lambda-1 and the turret protein lambda-2 form the star core, followed by the stepwise attachment of sigma-2 that completes the core and mu1/sigma3 that complete the outer layer. Binds to the host neuronal receptor RTN4R for viral entry into host neural cells. 1. Yue, Z. et al. (1997) Virology 234, 364-71. PMID: 9268168 2. Liu, X. et al. (2024) Nature communications 15, 10638. PMID: 39639006 3. Sutherland, DM. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2219404120. PMID: 37276413
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant MRV-3 Sigma3/S4 Protein, N-His

Referencias

Datasheet

MRV-3 Sigma3/S4 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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