ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Mycoplasma pneumoniae
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
69.15 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
ADP-ribosylating toxin CARDS is a ~68 kDa protein. The main virulence factor for this bacteria, a mono-ADP-ribosylating toxin (mART), that transfers the ADP-ribosyl group from NAD(+) to multiple target proteins in vitro. Also elicits cytopathic effects in mammalian cells, such as disorganization and disruption of respiratory epithelial integrity in tracheal epithelium and vacuolization in the cytoplasm of CHO and HeLa cells as well as in mice and baboons. Treatment of mice or baboons with CARDS elicits a response that is consistent with human M.pneumoniae infections and mouse models of both infection and intoxication, suggesting that CARDS toxin is sufficient to cause prolonged inflammatory responses and airway dysfunction. Treatment of baboons with CARDS induces a number of cytokines; G-CSF (40 fold), IL-1Ra (10 fold), IL-6 and IL-8 (333 and 100 fold, respectively), MIP-1a (5 fold), and RANTES (9 fold). Treatment of mice gives a similar response.
1. Kannan, TR. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6724-9. PMID: 16617115
2. Kannan, TR. et al. (2014) Molecular microbiology 93, 568-81. PMID: 24948331
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