

| Каталожный номер | JN079012 |
|---|---|
| Описание |
Recombinant Mycoplasma pneumoniae Mgp3/P90 Protein, N-His (JN079012) expressed in E. coli, spanning Asn467-Gly688. Purity: >90% by SDS-PAGE.
Highlights
|
| Система экспрессии | E. coli |
| Номер доступа | Q50341 |
| Длина белка | Asn467-Gly688 |
| Применения | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Виды | Mycoplasma pneumoniae |
| Характер | Recombinant |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >90% as determined by SDS-PAGE. |
| Прогнозируемая молекулярная масса | 26.08 kDa |
| Форма | Lyophilized |
| Буфер хранения | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Реконституция | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Доставка | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Альтернативные названия | Mgp-operon protein 3, Mgp3, ORF-3 protein, MPN_142, MP012 |
| Фон | Adhesin P40/P90 (Mgp3) is a ~130 kDa protein. Part of major adhesin Nap complex of the attachment organelle which mediates attachment to host ciliated epithelium and plays a major role in gliding motility. Binds sialylated oligosaccharides, which are present on the surface of its human host cells and which probably serve as the main host cell receptors for this bacterium. This protein and P1 mutually stabilize each other, and are required for formation of the attachment organelle. 1. Sperker, B. et al. (1991) Molecular microbiology 5, 299-306. PMID: 1904124 2. Vizarraga, D. et al. (2020) Nature communications 11, 5188. PMID: 33057023 3. Vizarraga, D. et al. (2025) PLoS pathogens 21, e1012973. PMID: 40153444 4. Marseglia, A. et al. (2024) International journal of biological macromolecules 279, 135277. PMID: 39226978 |
| Примечание | For research use only. |

SDS-PAGE for Recombinant Mycoplasma pneumoniae Mgp3/P90 Protein, N-His
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