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Nipah virus/NiV Recombinant Protein N/Nucleoprotein Protein (N-His-SUMO & C-Strep) (VK432022)

Nipah virus/NiV Recombinant Protein N/Nucleoprotein Protein (N-His-SUMO & C-Strep)
Nipah virus/NiV Recombinant Protein N/Nucleoprotein Protein (N-His-SUMO & C-Strep)
Precio(USD):
Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
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  • Datasheet

  • SDS

Vista general
Número de catálogoVK432022
Descripción
Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His-SUMO & C-Strep (VK432022) expressed in E. coli, spanning Thr32-Gly402. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoQ9IK92
Longitud de proteínaThr32-Gly402
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesNipah virus (NiV)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 54.71 kDa
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosNucleoprotein, Protein N, Nucleocapsid protein, N
Fondo

Nucleoprotein (Protein N) is a ~58 kDa protein. Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 13.4 N per turn, approximately 20 nm in diameter, with a hollow central cavity of 56 nm in diameter. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P.

1. Ker, DS. et al. (2021) PLoS pathogens 17, e1009740. PMID: 34270629
2. Yabukarski, F. et al. (2014) Nature structural & molecular biology 21, 754-9. PMID: 25108352
Nota For research use only.
Imágenes
  • Nipah virus/NiV Recombinant Protein N/Nucleoprotein Protein (N-His-SUMO & C-Strep)

    SDS-PAGE

    SDS-PAGE for Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His-SUMO & C-Strep

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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