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| カタログ番号 | RW476012 |
|---|---|
| 説明 |
Recombinant Rat DIO1 Protein, N-His (RW476012) expressed in E. coli, spanning Arg85-Phe257. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P24389 |
| タンパク質長 | Arg85-Phe257 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Rattus norvegicus (Rat) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 21.85 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | 5DI, DIO1, DIOI, EC:1.21.99.3, EC:1.21.99.4, ITDI1, TXDI1, Type 1 DI, Type I iodothyronine deiodinase, Type-I 5'-deiodinase |
| 背景 | Type I iodothyronine deiodinase is a ~29 kDa protein. Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination. Catalyzes the deiodination of L-thyroxine (T4) to 3,5,3'-triiodothyronine (T3) and 3',5'-diiodothyronine (3',5'-T2) to 3'-monoiodothyronine (3'-T1) via outer-ring deiodination (ORD). Catalyzes the deiodination of T4 to 3,3',5'-triiodothyronine (rT3), 3,5-diiodothyronine (3,5-T2) to 3-monoiodothyronine(3-T1) and 3,3'-diiodothyronine (3,3'-T2) to 3-T1 via inner-ring deiodination (IRD). Catalyzes the deiodination of rT3 to 3,3'-T2 via ORD. Catalyzes the phenolic ring deiodinations of 3,3',5'-triiodothyronamine, 3',5'-diiodothyronamine and 3,3'-diiodothyronamine as well as tyrosyl ring deiodinations of 3,5,3'-triiodothyronamine and 3,5-diiodothyronamine. 1. Berry, MJ. (1992) The Journal of biological chemistry 267, 18055-9. PMID: 1517238 2. Leonard, JL. et al. (1991) The Journal of biological chemistry 266, 11262-9. PMID: 2040632 3. Toyoda, N. et al. (1995) The Journal of biological chemistry 270, 12310-8. PMID: 7744884 4. Berry, MJ. et al. (1991) Nature 349, 438-40. PMID: 1825132 5. Moreno, M. et al. (1994) FEBS letters 344, 143-6. PMID: 8187873 |
| 注意事項 | For research use only |
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