Renilla reniformis Rluc/Luciferase Recombinant Protein (N-His) (ZA998022)

Name: Renilla reniformis Rluc/Luciferase Recombinant Protein (N-His)
Brand: abinScience
SKU: ZA998022
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	ZA998022
Описание	Recombinant Renilla reniformis Rluc/Luciferase Protein, N-His (ZA998022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P27652
Длина белка	Met1-Gln311
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Renilla reniformis (Sea pansy)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Coelenterazine h 2-monooxygenase, EC:1.13.12.5, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase
Фон	Coelenterazine h 2-monooxygenase is a ~36 kDa protein. Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
Примечание	For research use only

Изображения

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Даташит

Renilla reniformis Rluc/Luciferase Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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