

| Numéro de catalogue | VK662011 |
|---|---|
| Description |
Recombinant SFTSV Mgp1/membrane glycoprotein polyprotein, C-His (VK662011) expressed in Mammalian Cells, spanning Gly19-Lys452. Purity: >90% by SDS-PAGE.
Highlights
|
| Système d'expression | Mammalian cells |
| Numéro d'accès | R4V2Q5 |
| Longueur de protéine | Gly19-Lys452 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Espèces | Dabie bandavirus (Severe fever with thrombocytopenia virus) (SFTSV) (Huaiyangshan banyangvirus) |
| Nature | Recombinant |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >90% as determined by SDS-PAGE. |
| Masse moléculaire prédite | 50.32 kDa |
| Forme | Lyophilized |
| Tampon de stockage | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Reconstitution | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Expédition | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Noms alternatifs | membrane glycoprotein polyprotein, membrane glycoprotein polyprotein |
| Contexte | Envelopment polyprotein (Mgp1/membrane glycoprotein polyprotein) is a ~116 kDa protein. Structural component of the virion that interacts with glycoprotein C. It shields the hydrophobic fusion loops of the glycoprotein C, preventing premature fusion. The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation. They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after clathrin-mediated endocytosis of the virion. Plays a role in the packaging of ribonucleoproteins during virus assembly. 1. Hofmann, H. et al. (2013) Journal of virology 87, 4384-94. PMID: 23388721 |
| Note | For research use only. |

SDS-PAGE for Recombinant SFTSV Mgp1/membrane glycoprotein polyprotein, C-His
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