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Toxoplasma gondii SAG1/Major surface antigen p30 Recombinant Protein (N-His-SUMO) (JN902012)

Toxoplasma gondii SAG1/Major surface antigen p30 Recombinant Protein (N-His-SUMO)
Toxoplasma gondii SAG1/Major surface antigen p30 Recombinant Protein (N-His-SUMO)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерJN902012
Описание
Recombinant Toxoplasma gondii SAG1/Major surface antigen p30 Protein, N-His-SUMO (JN902012) expressed in E. coli, spanning Ser48-Leu198. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииE. coli
Номер доступаP13664
Длина белкаSer48-Leu198
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыToxoplasma gondii
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 28.32 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияMajor surface antigen p30
Фон

Major surface antigen p30 (SAG1) is a ~34 kDa protein. Mediates binding to host cells and is especially important for parasite attachment and fitness in IFN-gamma/IFNG-stimulated host cells. May regulate binding between human S100A6 and vimentin (VIM) for cytoskeleton organization during the early stages of invasion into host cells. Promotes TNF secretion in host cells probably by enhancing binding between human VIM and PRKCQ and activating NF-kappa-B pathway. Promotes viability of host cells to satisfy the survival needs of parasites through the interaction with human RACK1.

1. Robinson, SA. et al. (2004) Parasitology 128, 391-6. PMID: 15151144
2. Cohen, O. et al. (2025) Infection and immunity 93, e0001025. PMID: 40607979
3. Zhou, LJ. et al. (2021) iScience 24, 103514. PMID: 34950858
4. Wang, D. et al. (2024) Acta tropica 251, 107112. PMID: 38157925
Примечание For research use only.
Изображения
  • Toxoplasma gondii SAG1/Major surface antigen p30 Recombinant Protein (N-His-SUMO)

    SDS-PAGE

    SDS-PAGE for Recombinant Toxoplasma gondii SAG1/Major surface antigen p30 Protein, N-His-SUMO

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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