VSV Nucleoprotein Recombinant Protein (N-His-SUMO) (VK422042)

Name: VSV Nucleoprotein Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: VK422042
Price: 211 USD
Availability: InStock

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Número de catálogo	VK422042
Descripción	Recombinant VSIV Nucleoprotein, N-His-SUMO (VK422042) expressed in E. coli, spanning Ser2-Lys422. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P03521
Longitud de proteína	Ser2-Lys422
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Vesicular stomatitis Indiana virus (VSIV)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	59.62 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo	Nucleoprotein is a ~47 kDa protein. Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. The nucleocapsid is bullet-shaped with the tip containing 8 turns of a conical spiral before reaching the helical cylindrical trunk. Nucleocapsid assembly is concomitant with replication, therefore viral replication depends on the intracellular concentration of free N, termed N(0). All replicative products are resistant to nucleases. 1. Green, TJ. et al. (2006) Science (New York, N.Y.) 313, 357-60. PMID: 16778022 2. Ge, P. et al. (2010) Science (New York, N.Y.) 327, 689-93. PMID: 20133572 3. Gumpper, RH. et al. (2018) Journal of virology 92. PMID: 29437970 4. Jenni, S. et al. (2022) Nature communications 13, 4802. PMID: 35970826 5. Zhou, K. et al. (2022) Nature communications 13, 5980. PMID: 36216930 6. Green, TJ. et al. (2011) Journal of virology 85, 2714-22. PMID: 21177817 7. Si, Z. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2111948119. PMID: 35476516
Nota	For research use only