Anti-Human 5'-Nucleotidase/CD73 Reference Antibody (Ansipastobart, RUO) (HB209156)

Name: Anti-Human 5'-Nucleotidase/CD73 Reference Antibody (Ansipastobart, RUO)
Brand: abinScience
SKU: HB209156
Price: 176 USD
Availability: InStock

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100ug
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Aperçu
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HB209156

Description

Ansipastobart (HB209156) is a research-grade recombinant antibody targeting CD73. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

Reactivité des espèces

Human

Applications

ELISA, Bioactivity: FCM, Functional assay, Research in vivo, WB

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG1-lambda2

Applications testées

Cible

5'-NT, 5'-deoxynucleotidase, 5'-nucleotidase, CD73, EC:3.1.3.35, EC:3.1.3.5, EC:3.1.3.89, EC:3.1.3.91, EC:3.1.3.99, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, NT5, NT5E, NTE, Thymidylate 5'-phosphatase

Niveau d'endotoxines

< 10 EU/mg

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P21589

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Noms alternatifs

2829250-32-8

Contexte

5'-nucleotidase (CD73/NT5E) is a ~63 kDa protein. Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.

1. Garavaglia, S. et al. (2012) The Biochemical journal 441, 131-41. PMID: 21933152

2. Heuts, DP. et al. (2012) Chembiochem : a European journal of chemical biology 13, 2384-91. PMID: 22997138

3. Knapp, K. et al. (2012) Structure (London, England : 1993) 20, 2161-73. PMID: 23142347

4. Fausther, M. et al. (2014) PloS one 9, e98568. PMID: 24887587

5. Scaletti, E. et al. (2021) Purinergic signalling 17, 693-704. PMID: 34403084

Note

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1, lambda Isotype Control antibody (HyHEL-10) (HP248107)

Images

SDS-PAGE

SDS-PAGE for Research Grade Ansipastobart
Bioactivity

Detects recombinant CD73 (Catalog No: HB209011) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-CD73 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD73 (Catalog No: HB209011)
Lane 2: Negative control