Anti-Human 5'-Nucleotidase/CD73 Reference Antibody (Ansipastobart, RUO) (HB209156)

Name: Anti-Human 5'-Nucleotidase/CD73 Reference Antibody (Ansipastobart, RUO)
Brand: abinScience
SKU: HB209156
Price: 176 USD
Availability: InStock

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100ug
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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HB209156

説明

Ansipastobart (HB209156) is a research-grade recombinant antibody targeting CD73. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

種反応性

Human

アプリケーション

ELISA, Bioactivity: FCM, Functional assay, Research in vivo, WB

宿主種

Human

クローン性

Monoclonal

アイソタイプ

IgG1-lambda2

テスト済みアプリケーション

ターゲット

5'-NT, 5'-deoxynucleotidase, 5'-nucleotidase, CD73, EC:3.1.3.35, EC:3.1.3.5, EC:3.1.3.89, EC:3.1.3.91, EC:3.1.3.99, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, NT5, NT5E, NTE, Thymidylate 5'-phosphatase

エンドトキシンレベル

< 10 EU/mg

純度

>95% as determined by SDS-PAGE.

精製

Protein A/G purified from cell culture supernatant.

アクセッション番号

P21589

形態

Liquid

保存バッファー

0.01M PBS pH 7.4.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

別名

2829250-32-8

背景

5'-nucleotidase (CD73/NT5E) is a ~63 kDa protein. Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN.

1. Garavaglia, S. et al. (2012) The Biochemical journal 441, 131-41. PMID: 21933152

2. Heuts, DP. et al. (2012) Chembiochem : a European journal of chemical biology 13, 2384-91. PMID: 22997138

3. Knapp, K. et al. (2012) Structure (London, England : 1993) 20, 2161-73. PMID: 23142347

4. Fausther, M. et al. (2014) PloS one 9, e98568. PMID: 24887587

5. Scaletti, E. et al. (2021) Purinergic signalling 17, 693-704. PMID: 34403084

注意事項

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1, lambda Isotype Control antibody (HyHEL-10) (HP248107)

イメージ

SDS-PAGE

SDS-PAGE for Research Grade Ansipastobart
Bioactivity

Detects recombinant CD73 (Catalog No: HB209011) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-CD73 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD73 (Catalog No: HB209011)
Lane 2: Negative control