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Anti-Human Complement C5a Reference Antibody (MEDI7814, RUO) (HF687136)

Anti-Human Complement C5a Reference Antibody (MEDI7814, RUO)
Anti-Human Complement C5a Reference Antibody (MEDI7814, RUO)
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  • 概要

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  • データシート

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概要
カタログ番号HF687136
種反応性Human
アプリケーションELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種Human
クローン性Monoclonal
アイソタイプIgG
クローンIDMEDI7814
発現システム Mammalian cells
ターゲット CPAMD4, C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, Complement C5
エンドトキシンレベル < 10 EU/mg
純度 >95% purity as determined by SDS-PAGE.
精製 Protein A/G purified from cell culture supernatant.
アクセッション番号 P01031
形態 Liquid
保存バッファー 0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名MEDI7814
背景

Complement C5 (C5a/C5a anaphylatoxin) is a ~188 kDa protein. Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Activated downstream of classical, alternative, lectin and GZMK complement pathways.

1. Rawal, N. et al. (2003) The Journal of biological chemistry 278, 38476-83. PMID: 12878586
2. Rawal, N. et al. (2008) The Journal of biological chemistry 283, 7853-63. PMID: 18204047
3. Heesterbeek, DA. et al. (2019) The EMBO journal 38. PMID: 30643019
4. DiScipio, RG. et al. (1983) The Journal of biological chemistry 258, 10629-36. PMID: 6554279
5. Donado, CA. et al. (2025) Nature 641, 211-221. PMID: 39914456
6. Lan, F. et al. (2025) Nature 638, 490-498. PMID: 39814882
参考文献 1. Colley CS, et al. Structure and characterization of a high affinity C5a monoclonal antibody that blocks binding to C5aR1 and C5aR2 receptors. MAbs. 2018 Jan;10(1):104-117. [HF687136]
注意事項 For research use only. Not suitable for clinical or therapeutic use.
イメージ
  • Anti-Human Complement C5a Reference Antibody (MEDI7814, RUO)

    SDS-PAGE

    SDS-PAGE for Research Grade Anti-Human C5a (MEDI7814)

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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