中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HB016046 |
|---|---|
| 種反応性 | Human |
| アプリケーション | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 宿主種 | Human |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1-kappa |
| クローンID | GSK2618960 |
| 生物学的活性 | GSK2618960 (10-100 ug/mL; 10 h) potently induces dose-dependent CD4 + T cell proliferation in PBMCs [2]. GSK2618960 binds specifically to monocyte-derived DCs, but not to uncultured primary DCs, NK cells, or B cells from fresh PBMCs [2]. GSK2618960 (50 ug/mL; 7 days) enhances the differentiation of DCs and upregulates their activation marker (CD83, CD86 and CD209) expression in PBMCs [2]. |
| 発現システム | Mammalian cells |
| テスト済みアプリケーション | FCM |
| ターゲット | CD127, CDw127, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, Interleukin-7 receptor subunit alpha |
| エンドトキシンレベル | < 10 EU/mg |
| 純度 | >95% purity as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P16871 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | GSK2618960 |
| 背景 | Interleukin-7 receptor subunit alpha (CD127/IL7R) is a ~51 kDa protein. Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). |
| 参考文献 | 1. Ellis J, et al. Anti-IL-7 receptor α monoclonal antibody (GSK2618960) in healthy subjects - a randomized, double-blind, placebo-controlled study. Br J Clin Pharmacol. 2019;85(2):304-315. [HB016046] 2. Liao K, et al. Characterization of the robust humoral immune response to GSK2618960, a humanized anti-IL-7 receptor monoclonal antibody, observed in healthy subjects in a Phase 1 study. PLoS One. 2021;16(3):e0249049. Published 2021 Mar 23. [HB016046] |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) Human IgG1 (E356D/M358L), kappa Recombinant Isotype Control Antibody (HyHEL-10) (HV080013) Anti-LASV GPC Recombinant Antibody (Fab 37.7) (VK453013) |
Detects Human CD127/IL7R in indirect ELISAs.
SDS-PAGE for Research Grade Anti-Human CD127/IL7R (GSK2618960)
Flow-cytometry using APC anti-human CD127 antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD127 monoclonal antibody (Catalog HB016046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human CD127 antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD127 monoclonal antibody (Catalog HB016046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
