Anti-Human MCAM/CD146 Reference Antibody (Abx-Ma1, RUO) (HW484026)

Name: Anti-Human MCAM/CD146 Reference Antibody (Abx-Ma1, RUO)
Brand: abinScience
SKU: HW484026
Price: 176 USD
Availability: InStock

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100ug
1mg
5mg
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50mg
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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HW484026
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1
クローンID	Abx-Ma1
発現システム	Mammalian cells
テスト済みアプリケーション	FCM
ターゲット	CD146, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, MUC18, MCAM, Melanoma-associated antigen MUC18, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P43121
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	C3.19.1, Abx-Ma1
背景	Cell surface glycoprotein MUC18 (CD146/MCAM) is a ~71 kDa protein. Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
参考文献	1. Braeuer RR, et al. Transcriptional control of melanoma metastasis: the importance of the tumor microenvironment. Semin Cancer Biol. 2011 Apr;21(2):83-8. [HW484026]
注意事項	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

イメージ

SEC-HPLC

SEC-HPLC detection for Research Grade Anti-Human CD146/MUC18/MCAM Antibody (Abx-Ma1).
Bioactivity

Detects CD146/MUC18/MCAM in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD146/MUC18/MCAM Antibody (Abx-Ma1).
Flow CytoMetry

Flow-cytometry using APC anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.