Anti-Human BTLA/CD272 Reference Antibody (ANB032, RUO) (HV375036)

Name: Anti-Human BTLA/CD272 Reference Antibody (ANB032, RUO)
Brand: abinScience
SKU: HV375036
Price: 176 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HV375036
Reactivité des espèces	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG4-kappa
ID de clone	ANB032
Activité biologique	ANB032 reduces dendritic cell maturation in in vitro assays. ANB032 reduces T cell proliferation and secretion of IFN-γ, IL-5, IL-13, and IL-17A, and downregulates BTLA expression during T cell stimulation in PBMCs [1]. ANB032 reduces T cell expansion, plasma inflammatory cytokines, and demonstrates in vivo efficacy in the humanized GvHD mouse model [1].
Système d'expression	Mammalian cells
Cible	B- and T-lymphocyte attenuator, BTLA, CD272, B- and T-lymphocyte-associated protein
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	Q7Z6A9
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	ANB032
Contexte	B- and T-lymphocyte attenuator (CD272/BTLA) is a ~32 kDa protein. Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells. 1. Watanabe, N. et al. (2003) Nature immunology 4, 670-9. PMID: 12796776 2. Gavrieli, M. et al. (2003) Biochemical and biophysical research communications 312, 1236-43. PMID: 14652006 3. Sedy, JR. et al. (2005) Nature immunology 6, 90-8. PMID: 15568026 4. Cai, G. et al. (2008) Nature immunology 9, 176-85. PMID: 18193050 5. Cheung, TC. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 183, 7286-96. PMID: 19915044
Reférences	1. Ken Luu, et al. ANB032, a novel BTLA agonist monoclonal antibody, inhibited T cell proliferation, reduced inflammatory cytokines, and down modulated BTLA expression on circulating T and B cells in phase 1, progresses into a phase 2 study in atopic dermatitis. Journal of Investigative Dermatology, 143S106. [HV375036]
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG4 (S228P) Recombinant Isotype Control Antibody (SAA0184) (HP298107)

Images

Bioactivity

Detects Recombinant Human CD272/BTLA Protein, C-His (Cat No.: HV375011) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD272/BTLA (ANB032).
Flow cytometry

Flow-cytometry APC using anti-human CD272 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD272 monoclonal antibody (Catalog HV375036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry PE using anti-human CD272 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human CD272 monoclonal antibody (Catalog HV375036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry FITC using anti-human CD272 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human CD272 monoclonal antibody (Catalog HV375036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.