Anti-Human CD30/TNFRSF8 Reference Antibody (HeFi-1, RUO) (HB038056)

Name: Anti-Human CD30/TNFRSF8 Reference Antibody (HeFi-1, RUO)
Brand: abinScience
SKU: HB038056
Price: 370 USD
Availability: InStock

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概要
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参考文献
データシート
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概要

カタログ番号	HB038056
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG
クローンID	HeFi-1
生物学的活性	HeFi-1 (0.1 ng/uL, 1 h) can bind specifically to Karpas299 cells with a binding rate of over 90 % [1]. HeFi-1 (20 ug/mL, 48 h) can significantly inhibit the proliferation of Karpas299 cells and induces G1 phase blockade [1]. HeFi-1 (20 ug/mL, 12-24 h) can significantly induce apoptosis in eosinophil [2]. Cell Proliferation Assay [1] Cell Line: Karpas299 cells Concentration: 20 ug/mL Incubation Time: 48 h Result: Significantly inhibited cell proliferation with an inhibition rate of 40%, superior to Daclizumab (). Cell Cycle Analysis [1] Cell Line: Karpas299 cells Concentration: 20 ug/mL Incubation Time: 48 h Result: Induced G1 phase arrest and a decrease in S phase cells. HeFi-1 (100 ug, i.v., once weekly, for 4 weeks) can effectively prolong survival in SCID/NOD wild-type and FcRγ -/- mice bearing Karpas299 lymphoma [1].
発現システム	Mammalian cells
ターゲット	CD30L receptor, Ki-1 antigen, CD30, TNFRSF8, D1S166E, Tumor necrosis factor receptor superfamily member 8, Lymphocyte activation antigen CD30
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P28908
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	HeFi-1, HeFi1, HeFi1
背景	Tumor necrosis factor receptor superfamily member 8 (CD30/TNFRSF8) is a ~63 kDa protein. Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. CD30 is the therapeutic target of brentuximab vedotin (Adcetris). 1. Smith, CA. et al. (1993) Cell 73, 1349-60. PMID: 8391931 2. Aizawa, S. et al. (1997) The Journal of biological chemistry 272, 2042-5. PMID: 8999898
参考文献	1. Zhang M, et al. Effective therapy for a murine model of human anaplastic large-cell lymphoma with the anti-CD30 monoclonal antibody, HeFi-1, does not require activating Fc receptors. Blood. 2006 Jul 15;108(2):705-10. [HB038056] 2. Berro AI, et al. Increased expression and activation of CD30 induce apoptosis in human blood eosinophils. J Immunol. 2004 Aug 1;173(3):2174-83. [HB038056]
注意事項	For research use only. Not suitable for clinical or therapeutic use.