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| カタログ番号 | HY105026 |
|---|---|
| 種反応性 | Human |
| アプリケーション | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 宿主種 | Human |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1-kappa |
| クローンID | OM-RCA-01 |
| 生物学的活性 | OM-RCA-01 binds to FGFR1 with high affinity (K d = 1.59 nM) and inhibits its in vitro kinase activity [1]. OM-RCA-01 (1-100 ug/mL, 3 h) potently inhibits FGFR1 phosphorylation and cell proliferation in FGFR1-overexpressing human lung cancer cell line A549 (GI 50 = 9.69 ug/mL), but exerts no significant effect on FGFR1-low-expressing human melanoma cell line Mel Kor [1]. OM-RCA-01 (0.5-10 nmol/L; 5 days) enhances Nivolumab -induced IFN-γ release and T-cell proliferation in a human allogeneic MLR assay, whereas it exhibits minimal effects when used alone when combined with Nivolumab () [1]. OM-RCA-01 (1 ug/mL; 4 days) increases Nivolumab -induced IL-2 secretion by 29%-74% in human peripheral blood mononuclear cells (PBMCs) stimulated with Staphylococcal enterotoxin B (SEB) () [1]. Western Blot Analysis [1] Cell Line: A549 cells Concentration: 1-100ug/mL Incubation Time: 3 h Result: Resulted in a dose-dependent suppression of FGFR1 phosphorylation. Parmacokinetics Species Dose Route C 0 T 1/2 V d CL AUC 0-t AUC 0-∞ MRT Rat [1] 10 mg/kg i.v. 186 ng/mL 121 h 532 L 3.05 L/h 10665 ng·h/mL 32818 ng·h/mL 34 h Rat [1] 30 mg/kg i.v. 177 ng/mL 136 h 2533 L 12.88 L/h 14237 ng·h/mL 46602 ng·h/mL 34 h Rat [1] 100 mg/kg i.v. 245 ng/mL 234 h 7569 L 22.44 L/h 16383 ng·h/mL 89131 ng·h/mL 35 h OM-RCA-01 (30 mg/kg; i.v.; twice weekly) induces a statistically significant two-fold reduction in median tumor volume and delays tumor growth in A549 lung cancer xenograft models, and combination with Pembrolizumab () achieves 89% tumor growth inhibition [1]. OM-RCA-01 (30 mg/kg; once every 3 days) achieves 96% tumor growth delay in the cancer-associated fibroblast (CAF)-positive renal cell carcinoma cohort and 75% tumor growth delay in the CAF-negative renal cell carcinoma cohort, while increasing IFN-γ and IL-2 cytokine levels [1]. |
| 発現システム | Mammalian cells |
| ターゲット | FLT2, HBGFR, BFGFR, FGFR-1, CEK, Fibroblast growth factor receptor 1, FGFR1, bFGF-R-1, Fms-like tyrosine kinase 2, FGFBR, FLT-2, CD331, Basic fibroblast growth factor receptor 1, FLG, Proto-oncogene c-Fgr, N-sam |
| エンドトキシンレベル | < 10 EU/mg |
| 純度 | >95% purity as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P11362 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | OM-RCA-01 |
| 背景 | Fibroblast growth factor receptor 1 (CD331/FGFR1) is a ~91 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. |
| 参考文献 | 1. Tsimafeyeu I, et al. A novel anti-FGFR1 monoclonal antibody OM-RCA-01 exhibits potent antitumor activity and enhances the efficacy of immune checkpoint inhibitors in lung cancer models. Immunooncol Technol. 2024;23:100725. Published 2024 Jul 26. [HY105026] |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
SDS-PAGE for Research Grade Anti-Human CD331/FGFR1 (OM-RCA-01).
Flow-cytometry using PE anti-human CD331 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD331 monoclonal antibody (Catalog: HY105026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
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