Anti-Human FGFR1 Reference Antibody (OM-RCA-01, RUO) (HY105026)

Name: Anti-Human FGFR1 Reference Antibody (OM-RCA-01, RUO)
Brand: abinScience
SKU: HY105026
Price: 176 USD
Availability: InStock

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Обзор

Каталожный номер	HY105026
Реактивность видов	Human
Применения	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG1-kappa
ID клона	OM-RCA-01
Биологическая активность	OM-RCA-01 binds to FGFR1 with high affinity (K d = 1.59 nM) and inhibits its in vitro kinase activity [1]. OM-RCA-01 (1-100 ug/mL, 3 h) potently inhibits FGFR1 phosphorylation and cell proliferation in FGFR1-overexpressing human lung cancer cell line A549 (GI 50 = 9.69 ug/mL), but exerts no significant effect on FGFR1-low-expressing human melanoma cell line Mel Kor [1]. OM-RCA-01 (0.5-10 nmol/L; 5 days) enhances Nivolumab -induced IFN-γ release and T-cell proliferation in a human allogeneic MLR assay, whereas it exhibits minimal effects when used alone when combined with Nivolumab () [1]. OM-RCA-01 (1 ug/mL; 4 days) increases Nivolumab -induced IL-2 secretion by 29%-74% in human peripheral blood mononuclear cells (PBMCs) stimulated with Staphylococcal enterotoxin B (SEB) () [1]. Western Blot Analysis [1] Cell Line: A549 cells Concentration: 1-100ug/mL Incubation Time: 3 h Result: Resulted in a dose-dependent suppression of FGFR1 phosphorylation. Parmacokinetics Species Dose Route C 0 T 1/2 V d CL AUC 0-t AUC 0-∞ MRT Rat [1] 10 mg/kg i.v. 186 ng/mL 121 h 532 L 3.05 L/h 10665 ng·h/mL 32818 ng·h/mL 34 h Rat [1] 30 mg/kg i.v. 177 ng/mL 136 h 2533 L 12.88 L/h 14237 ng·h/mL 46602 ng·h/mL 34 h Rat [1] 100 mg/kg i.v. 245 ng/mL 234 h 7569 L 22.44 L/h 16383 ng·h/mL 89131 ng·h/mL 35 h OM-RCA-01 (30 mg/kg; i.v.; twice weekly) induces a statistically significant two-fold reduction in median tumor volume and delays tumor growth in A549 lung cancer xenograft models, and combination with Pembrolizumab () achieves 89% tumor growth inhibition [1]. OM-RCA-01 (30 mg/kg; once every 3 days) achieves 96% tumor growth delay in the cancer-associated fibroblast (CAF)-positive renal cell carcinoma cohort and 75% tumor growth delay in the CAF-negative renal cell carcinoma cohort, while increasing IFN-γ and IL-2 cytokine levels [1].
Система экспрессии	Mammalian cells
Цель	FLT2, HBGFR, BFGFR, FGFR-1, CEK, Fibroblast growth factor receptor 1, FGFR1, bFGF-R-1, Fms-like tyrosine kinase 2, FGFBR, FLT-2, CD331, Basic fibroblast growth factor receptor 1, FLG, Proto-oncogene c-Fgr, N-sam
Уровень эндотоксинов	< 10 EU/mg
Чистота	>95% purity as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P11362
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	OM-RCA-01
Фон	Fibroblast growth factor receptor 1 (CD331/FGFR1) is a ~91 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate.
Ссылки	1. Tsimafeyeu I, et al. A novel anti-FGFR1 monoclonal antibody OM-RCA-01 exhibits potent antitumor activity and enhances the efficacy of immune checkpoint inhibitors in lung cancer models. Immunooncol Technol. 2024;23:100725. Published 2024 Jul 26. [HY105026]
Примечание	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Изображения

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD331/FGFR1 (OM-RCA-01).
Flow CytoMetry

Flow-cytometry using PE anti-human CD331 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD331 monoclonal antibody (Catalog: HY105026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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Даташит

Anti-Human FGFR1 Reference Antibody (OM-RCA-01, RUO).pdf

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