Anti-Human FGFR2 Reference Antibody (HuGAL-FR21, RUO) (HB863056)

Name: Anti-Human FGFR2 Reference Antibody (HuGAL-FR21, RUO)
Brand: abinScience
SKU: HB863056
Price: 176 USD
Availability: InStock

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Numéro de catalogue	HB863056
Reactivité des espèces	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG
ID de clone	HuGAL-FR21
Activité biologique	HuGAL-FR21 (0.001-1 ug/mL) can specifically bind to FGFR2IIIb (EC 50 approximately 20 pM) and its mutant S252W in SNU-16 cells [1]. HuGAL-FR21 (0.01-100 ug/mL) effectively blocks the binding of FGFR2IIIb (IC 50 approximately 1.5-3 ug/mL) to FGF2, FGF7, and FGF10 [1]. HuGAL-FR21 (10 ug/mL, 30 min) effectively blocks FGF7 induced phosphorylation and significantly inhibits FGF2 induced phosphorylation in SNU-16 cells [1]. HuGAL-FR21 (10 ug/mL, 2-24 h) downregulates FGFR2 protein levels in SNU-16 cells [1]. Western Blot Analysis [1] Cell Line: SNU-16 cells Concentration: 10 ug/mL Incubation Time: 2, 8 and 48 h Result: Reduced the level of FGFR2 protein by approximately 50%. HuGAL-FR21 (0.5-5 mg/kg; i.p.; twice weekly; for 25-30 days) effectively inhibits the growth of tumors and has no obvious toxicity in athymic nude mice bearing SNU-16 xenografts or OCUM-2M xenografts [1].
Système d'expression	Mammalian cells
Cible	K-sam, KSAM, CD332, Keratinocyte growth factor receptor, Fibroblast growth factor receptor 2, FGFR-2, FGFR2, KGFR, BEK
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P21802
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	HuGAL-FR21
Contexte	Fibroblast growth factor receptor 2 (CD332/FGFR2) is a ~92 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4.
Reférences	1. Zhao WM, et al. Monoclonal antibodies to fibroblast growth factor receptor 2 effectively inhibit growth of gastric tumor xenografts. Clin Cancer Res. 2010 Dec 1;16(23):5750-8. [HB863056]
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)