Anti-Human FGFR2 Reference Antibody (HuGAL-FR21, RUO) (HB863056)

Name: Anti-Human FGFR2 Reference Antibody (HuGAL-FR21, RUO)
Brand: abinScience
SKU: HB863056
Price: 176 USD
Availability: InStock

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概要
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概要

カタログ番号	HB863056
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG
クローンID	HuGAL-FR21
生物学的活性	HuGAL-FR21 (0.001-1 ug/mL) can specifically bind to FGFR2IIIb (EC 50 approximately 20 pM) and its mutant S252W in SNU-16 cells [1]. HuGAL-FR21 (0.01-100 ug/mL) effectively blocks the binding of FGFR2IIIb (IC 50 approximately 1.5-3 ug/mL) to FGF2, FGF7, and FGF10 [1]. HuGAL-FR21 (10 ug/mL, 30 min) effectively blocks FGF7 induced phosphorylation and significantly inhibits FGF2 induced phosphorylation in SNU-16 cells [1]. HuGAL-FR21 (10 ug/mL, 2-24 h) downregulates FGFR2 protein levels in SNU-16 cells [1]. Western Blot Analysis [1] Cell Line: SNU-16 cells Concentration: 10 ug/mL Incubation Time: 2, 8 and 48 h Result: Reduced the level of FGFR2 protein by approximately 50%. HuGAL-FR21 (0.5-5 mg/kg; i.p.; twice weekly; for 25-30 days) effectively inhibits the growth of tumors and has no obvious toxicity in athymic nude mice bearing SNU-16 xenografts or OCUM-2M xenografts [1].
発現システム	Mammalian cells
ターゲット	K-sam, KSAM, CD332, Keratinocyte growth factor receptor, Fibroblast growth factor receptor 2, FGFR-2, FGFR2, KGFR, BEK
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P21802
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	HuGAL-FR21
背景	Fibroblast growth factor receptor 2 (CD332/FGFR2) is a ~92 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4.
参考文献	1. Zhao WM, et al. Monoclonal antibodies to fibroblast growth factor receptor 2 effectively inhibit growth of gastric tumor xenografts. Clin Cancer Res. 2010 Dec 1;16(23):5750-8. [HB863056]
注意事項	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)