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| カタログ番号 | HC359326 |
|---|---|
| 説明 |
Anti-Human CD47 Reference Antibody (ZL-1201, RUO) (HC359326) is a human monoclonal antibody detecting CD47.
|
| 種反応性 | Human |
| アプリケーション | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
| 宿主種 | Human |
| クローン性 | Monoclonal |
| アイソタイプ | IgG |
| クローンID | ZL-1201 |
| 生物学的活性 | ZL-1201 (15 min) binds to CD47 on Jurkat cells [1]. ZL-1201 (10 ug/mL; 30 min) when combined with Trastuzumab (), Cetuximab (), or Rituximab () significantly increases phagocytosis index compared to single agents or isotype control [1]. ZL-1201 (2 h) does not induce hemagglutination in whole blood [1]. ZL-1201 (10 mg/kg; i.p.; twice per week; 3–12 weeks) enhances antitumor efficacy in BALB/c nude mice with lymphoma, breast cancer, ovarian cancer, head and neck squamous cell carcinoma, and gastric cancer xenografts (with a TGI of >80% in Raji lymphoma xenografts) [1]. |
| ターゲット | Antigenic surface determinant protein OA3, CD47, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6 |
| エンドトキシンレベル | < 10 EU/mg as determined by LAL method. |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | Q08722 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | ZL-1201, ZL1201, ZL1201 |
| 背景 | Leukocyte surface antigen CD47 (MER6) is a ~35 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. CD47 is the therapeutic target of magrolimab (clinical). 1. Latour, S. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 2547-54. PMID: 11509594 2. Piccio, L. et al. (2005) Blood 105, 2421-7. PMID: 15383453 3. Isenberg, JS. et al. (2009) The Journal of biological chemistry 284, 1116-25. PMID: 19004835 4. Lindberg, FP. et al. (1993) The Journal of cell biology 123, 485-96. PMID: 7691831 5. Gao, AG. et al. (1996) The Journal of biological chemistry 271, 21-4. PMID: 8550562 6. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621 7. Ghimire, K. et al. (2020) Cells 9. PMID: 32679764 |
| 参考文献 | 1. Cao A, et al. CD47-blocking Antibody ZL-1201 Promotes Tumor-associated Macrophage Phagocytic Activity and Enhances the Efficacy of the Therapeutic Antibodies and Chemotherapy. Cancer Res Commun. 2022;2(11):1404-1417. Published 2022 Nov 10. [HC359326] |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
SDS-PAGE for Research Grade Anti-Human CD47/MER6 (ZL-1201)
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