Anti-Human CD9 Reference Antibody (AT14-012, RUO) (HB984016)

Name: Anti-Human CD9 Reference Antibody (AT14-012, RUO)
Brand: abinScience
SKU: HB984016
Price: 176 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HB984016
説明	Anti-Human CD9 (AT14-012) (HB984016) is a research-grade recombinant antibody targeting CD9. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1-kappa
クローンID	AT14-012
発現システム	Mammalian cells
テスト済みアプリケーション	FCM
ターゲット	Motility-related protein, Leukocyte antigen MIC3, TSPAN29, Cell growth-inhibiting gene 2 protein, CD9, MIC3, p24, Tspan-29, MRP-1, 5H9 antigen, Tetraspanin-29, CD9 antigen
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P21926
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	AT14-012, AT14012, AT14012
背景	CD9 antigen is a ~25 kDa protein. Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. 1. Higginbottom, A. et al. (2003) Biochemical and biophysical research communications 311, 208-14. PMID: 14575715 2. Nakazawa, Y. et al. (2008) Blood 112, 1730-9. PMID: 18541721 3. Ikeyama, S. et al. (1993) The Journal of experimental medicine 177, 1231-7. PMID: 8478605 4. Takeda, Y. et al. (2003) The Journal of cell biology 161, 945-56. PMID: 12796480 5. Masellis-Smith, A. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 2768-77. PMID: 7511626
注意事項	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

イメージ

Bioactivity

Detects Recombinant Human CD9 Protein, C-His (Cat No.: HB984021) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD9 (AT14-012).
Flow cytometry

Flow-cytometry using APC anti-human CD9 antibody. HCT-116 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD9 monoclonal antibody (Catalog HB984016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human CD9 antibody. HCT-116 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD9 monoclonal antibody (Catalog HB984016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human CD9 antibody. HCT-116 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD9 monoclonal antibody (Catalog HB984016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.