Anti-Human CD98 Reference Antibody (IGN523, RUO) (HY415026) is a human monoclonal antibody detecting CD98. Expressed in Mammalian cells.
Reactividad de especies
Human
Aplicaciones
ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Especie huésped
Human
Clonalidad
Monoclonal
Isotipo
IgG
ID de clon
IGN523
Actividad biológica
IGN523 elicits strong ADCC activity in Ramos, KG-1 and OCI-AML-3 cells, with EC 50 s of 18.6 ng/mL, 73.8 ng/mL and 83.3 ng/mL, respectively, does not reduce ADCC activity in IGN523 cells [1]. IGN523 (2 ug/mL, 0.5-48 h) elicits Caspase-dependent apoptosis, increases lysosomal membrane permeability and decreases amino acid transport in Ramos, KG-1 and OCI-AML-3 cells [1]. Apoptosis Analysis [1] Cell Line: Ramos, KG-1 and OCI-AML-3 cells Concentration: 2 ug/mL Incubation Time: 48 h Result: Decreased cell viability in the presence of AbXL (10 ug/mL) (Ramos: 70% reduction; OCI-AML-3: 76% reduction; KG-1: 80% reduction), but not when used alone. Increased caspase-3 and caspase-7 activity (Ramos: 3.3-fold; OCI-AML-3: 7.5-fold and KG-1: 6.6-fold). . IGN523 (15 mg/kg, i.p., once a week, twice/three times) inhibits tumor growth in Ramos and Dau xenografts mice model [1]. IGN523 (15 mg/kg, i.p., once a week, twice/three times/four times) inhibits tumor growth in AML and primary NSCLC xenograft mice models [1].
Sistema de expresión
Mammalian cells
Naturaleza
Recombinant
Objetivo
SLC3A2, 4F2hc, Lymphocyte activation antigen 4F2 large subunit, CD98, 4F2 cell-surface antigen heavy chain, Solute carrier family 3 member 2, MDU1, 4F2 heavy chain antigen
Nivel de endotoxinas
< 10 EU/mg as determined by LAL method.
Pureza
>95% as determined by SDS-PAGE.
Purificación
Protein A/G purified from cell culture supernatant.
Número de acceso
P08195
Forma
Liquid
Buffer de almacenamiento
0.01M PBS pH 7.4.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos
IGN523
Fondo
Amino acid transporter heavy chain SLC3A2 (CD98) is a ~57 kDa protein. Acts as a chaperone that facilitates biogenesis and trafficking of functional transporters heterodimers to the plasma membrane. Forms heterodimer with SLC7 family transporters (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10 and SLC7A11), a group of amino-acid antiporters. Heterodimers function as amino acids exchangers, the specificity of the substrate depending on the SLC7A subunit. Heterodimers SLC3A2/SLC7A6 or SLC3A2/SLC7A7 mediate the uptake of dibasic amino acids. Heterodimer SLC3A2/SLC7A11 functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane.
1. Rossier, G. et al. (1999) The Journal of biological chemistry 274, 34948-54. PMID: 10574970
2. Bröer, A. et al. (2000) The Biochemical journal 349 Pt 3, 787-95. PMID: 10903140
3. Yanagida, O. et al. (2001) Biochimica et biophysica acta 1514, 291-302. PMID: 11557028
4. Yan, R. et al. (2019) Nature 568, 127-130. PMID: 30867591
5. Yan, R. et al. (2020) Cell discovery 6, 82. PMID: 33298890
6. Yan, R. et al. (2021) Cell discovery 7, 16. PMID: 33758168
8. Mastroberardino, L. et al. (1998) Nature 395, 288-91. PMID: 9751058
9. Torrents, D. et al. (1998) The Journal of biological chemistry 273, 32437-45. PMID: 9829974
10. Pfeiffer, R. et al. (1999) The EMBO journal 18, 49-57. PMID: 9878049
Referencias
1. Hayes GM, Chinn L, Cantor JM, Cairns B, Levashova Z, Tran H, Velilla T, Duey D, Lippincott J, Zachwieja J, Ginsberg MH, H van der Horst E. Antitumor activity of an anti-CD98 antibody. Int J Cancer. 2015 Aug 1;137(3):710-20. [HY415026]
Nota
For research use only. Not suitable for clinical or therapeutic use.
Imágenes
SDS-PAGE
SDS-PAGE for Anti-Human CD98/SLC3A2 Reference Antibody (IGN523, RUO)
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