Anti-Human ErbB3/Her3 Reference Antibody (MP-RM-1, RUO) (HB116116)

Name: Anti-Human ErbB3/Her3 Reference Antibody (MP-RM-1, RUO)
Brand: abinScience
SKU: HB116116
Price: 176 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HB116116
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG
クローンID	MP-RM-1
生物学的活性	MP-RM-1 (0.01-10 ug/mL; 15-120 min) inhibits NRG-1β-dependent phosphorylation of ErbB-3 and Akt in a dose- and time-dependent manner in breast cancer cells MDA-MB-435 and melanoma cells IR-8 [1]. MP-RM-1 (10 ug/mL) significantly inhibits the colony-forming ability of IR-8 melanoma cells [1]. MP-RM-1 (20 mg/kg; i.p.; twice weekly) significantly inhibits the growth of ErbB-3 melanoma xenografts in nude mice, and reduces ErbB-3 expression and Akt phosphorylation levels in tumor tissues [1]. MP-RM-1 (20 mg/kg; i.p.; twice weekly) significantly inhibits the growth of DU-145 prostate cancer xenografts in nude mice [1].
発現システム	Mammalian cells
テスト済みアプリケーション	FCM
ターゲット	ERBB3, HER3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, Receptor tyrosine-protein kinase erbB-3
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P21860
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	MP-RM-1, MPRM1, MPRM1
背景	Receptor tyrosine-protein kinase erbB-3 (ERBB3/HER3) is a ~148 kDa protein. Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5. Involved in the regulation of myeloid cell differentiation. 1. Ieguchi, K. et al. (2010) The Journal of biological chemistry 285, 31388-98. PMID: 20682778 2. Kinugasa, Y. et al. (2004) Biochemical and biophysical research communications 321, 1045-9. PMID: 15358134 3. Braunstein, EM. et al. (2016) Leukemia 30, 2242-2245. PMID: 27416908
参考文献	1. Sala G, et al. An ErbB-3 antibody, MP-RM-1, inhibits tumor growth by blocking ligand-dependent and independent activation of ErbB-3/Akt signaling. Oncogene. 2012;31(10):1275-1286. [HB116116]
注意事項	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10) (HV080407) Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

イメージ

Bioactivity

Detects recombinant human ERBB3/Her3 (Cat No.: HB116011) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human ERBB3/HER3 (MP-RM-1).
Flow CytoMetry

Flow-cytometry using anti-human ERBB3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human ERBB3 monoclonal antibody (Catalog: HB116116) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.