Anti-Human ErbB3/Her3 Reference Antibody (MP-RM-1, RUO) (HB116116)

Name: Anti-Human ErbB3/Her3 Reference Antibody (MP-RM-1, RUO)
Brand: abinScience
SKU: HB116116
Price: 176 USD
Availability: InStock

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100ug
1mg
5mg
10mg
25mg
50mg
100mg

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Ссылки
Даташит
SDS

Обзор

Каталожный номер	HB116116
Реактивность видов	Human
Применения	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG
ID клона	MP-RM-1
Биологическая активность	MP-RM-1 (0.01-10 ug/mL; 15-120 min) inhibits NRG-1β-dependent phosphorylation of ErbB-3 and Akt in a dose- and time-dependent manner in breast cancer cells MDA-MB-435 and melanoma cells IR-8 [1]. MP-RM-1 (10 ug/mL) significantly inhibits the colony-forming ability of IR-8 melanoma cells [1]. MP-RM-1 (20 mg/kg; i.p.; twice weekly) significantly inhibits the growth of ErbB-3 melanoma xenografts in nude mice, and reduces ErbB-3 expression and Akt phosphorylation levels in tumor tissues [1]. MP-RM-1 (20 mg/kg; i.p.; twice weekly) significantly inhibits the growth of DU-145 prostate cancer xenografts in nude mice [1].
Система экспрессии	Mammalian cells
Проверенные приложения	FCM
Цель	ERBB3, HER3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, Receptor tyrosine-protein kinase erbB-3
Уровень эндотоксинов	< 10 EU/mg
Чистота	>95% purity as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P21860
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	MP-RM-1, MPRM1, MPRM1
Фон	Receptor tyrosine-protein kinase erbB-3 (ERBB3/HER3) is a ~148 kDa protein. Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5. Involved in the regulation of myeloid cell differentiation. 1. Ieguchi, K. et al. (2010) The Journal of biological chemistry 285, 31388-98. PMID: 20682778 2. Kinugasa, Y. et al. (2004) Biochemical and biophysical research communications 321, 1045-9. PMID: 15358134 3. Braunstein, EM. et al. (2016) Leukemia 30, 2242-2245. PMID: 27416908
Ссылки	1. Sala G, et al. An ErbB-3 antibody, MP-RM-1, inhibits tumor growth by blocking ligand-dependent and independent activation of ErbB-3/Akt signaling. Oncogene. 2012;31(10):1275-1286. [HB116116]
Примечание	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10) (HV080407) Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Изображения

Bioactivity

Detects recombinant human ERBB3/Her3 (Cat No.: HB116011) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human ERBB3/HER3 (MP-RM-1).
Flow CytoMetry

Flow-cytometry using anti-human ERBB3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human ERBB3 monoclonal antibody (Catalog: HB116116) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.