Anti-Human IL-10 Reference Antibody (MK-1966, RUO) (HB997036) is a human monoclonal antibody detecting IL-10.
Реактивность видов
Human
Применения
ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Хозяинский вид
Human
Клоональность
Monoclonal
Изотип
IgG
ID клона
MK-1966
Биологическая активность
MK-1966 strongly induces IFNγ production in human PBMCs when combined with the TLR9 agonist SD-101 [1]. MK-1966 (10 ug/mL; 24 h) restores CD8 + T-cell proliferation and functional cytokine production that is suppressed by COL4A1-induced M2-like macrophages [2].
Protein A/G purified from cell culture supernatant.
Номер доступа
P22301
Форма
Liquid
Буфер хранения
0.01M PBS pH 7.4.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
MK-1966, MK1966, MK1966
Фон
Interleukin-10 (IL10) is a ~20 kDa protein. Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.
1. Yoon, SI. et al. (2006) The Journal of biological chemistry 281, 35088-96. PMID: 16982608
2. El Kasmi, KC. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 7215-9. PMID: 18025162
3. Hashimoto, SI. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 3619-25. PMID: 11564774
4. de Waal Malefyt, R. et al. (1991) The Journal of experimental medicine 174, 1209-20. PMID: 1940799
5. Willems, F. et al. (1994) European journal of immunology 24, 1007-9. PMID: 7512027
6. Frei, K. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 2720-8. PMID: 8144879
Ссылки
1. Phan U, et al. Development of the anti-IL-10 mAb MK-1966 in combination with in situ vaccination of a TLR9 agonist SD-101 for cancer immunotherapy[J]. European Journal of Cancer, 2016, 69: S91-S92. [HB997036] 2. Chen J, et al. CD146+ CAFs mediate immunosuppression in gastric cancer via COL4A1: potential therapeutic targets. J Immunother Cancer. 2025;13(12):e013577. Published 2025 Dec 21. [HB997036]
Примечание
For research use only. Not suitable for clinical or therapeutic use.
SDS-PAGE for Research Grade Anti-Human IL10 (MK-1966)
Flow cytometry
Flow-cytometry using anti-human IL10 antibody. Transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL10 monoclonal antibody (Catalog HB997036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
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