Anti-Human IL-2 Reference Antibody (MT204, RUO) (HX011046) is a human monoclonal antibody detecting IL-2. Expressed in Mammalian cells.
Reactivité des espèces
Human
Applications
ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Espèce hôte
Human
Clonalité
Monoclonal
Isotype
IgG
ID de clone
MT204
Activité biologique
MT204 (1-100 ug/mL, 4 days) dose-dependently inhibits proliferation and IFN-γ secretion in rhesus PBMCs stimulated by the mitogen concavalin A [1]. MT204 can block CD25-bound IL-2 on high-affinity IL-2 receptors with a k d of 0.58 nM [1] [2]. MT204 (5 h) dose dependently reduces the IL-2 inducible cell surface expression of the alpha subunit of IL-4 receptor (CD124) in CTLL-2 cells [2]. MT204 (5 days) inhibits IL-2-induced proliferation of NKL cells and T cells with IC 50 s of approximately 1.3 nM and 0.21 nM, respectively [2]. MT204 (15 min) potently antagonizes downstream signaling of IL-2 at sub-nanomolar concentrations, significantly reducing cellular pY-STAT3 levels stimulated by IL-2 in Primary human lymphocytes [2]. MT204 significantly inhibits IL-2 induced proliferation independently of CD25 expression levels in NKL cells, T cells and all NK cells with IC 50 s of 0.35-0.36 nM, 0.33 nM, and 0.1-0.13 nM, respectively [2]. Parmacokinetics Species Dose Route Note Plasma Concentration T 1/2 Rhesus monkey 2, 10, 50 or repeat 50 mg/kg i.v. 1 h infusion 1 mg/mL 326.4 h MT204 (50 mg/kg, i.v., day 0, day 5 and day 12 at a speed of 1.5 mL/kg/h over 1 h) significant delays in rejection of allogeneic skin graft in allogeneic skin graft model of rhesus monkey [1].
Protein A/G purified from cell culture supernatant.
Numéro d'accès
P60568
Forme
Liquid
Tampon de stockage
0.01M PBS pH 7.4.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
MT204
Contexte
Interleukin-2 (IL2) is a ~17 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways.
6. Osinalde, N. et al. (2015) Proteomics 15, 520-31. PMID: 25142963
7. Holbrook, NJ. et al. (1984) Proceedings of the National Academy of Sciences of the United States of America 81, 1634-8. PMID: 6608729
Reférences
1. Plater-Zyberk C, et al. The interleukin-2 antagonizing antibody MT204 delays allogeneic skin graft rejection in non-human primates and is well tolerated. Transpl Immunol. 2011 Sep; 25(2-3):133-40. [HX011046] 2. Volkland J, et al. A humanized monoclonal antibody against interleukin-2 that can inactivate the cytokine/receptor complex. Mol Immunol. 2007 Mar; 44(7):1743-53. [HX011046]
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
SDS-PAGE
SDS-PAGE for Research Grade Anti-Human IL2 (MT204)
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