| Numéro de catalogue | HS892016 |
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| Reactivité des espèces | Human |
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| Applications | ELISA, WB, Bioactivity: FCM, Functional assay, Research in vivo |
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| Espèce hôte | Human |
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| Clonalité | Monoclonal |
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| Isotype | scFv-Fc |
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| ID de clone | ZEB85 |
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| Activité biologique |
ZEB85 (10-100 nM; 4 days) increases neurite complexity in developing primary mouse hippocampal neurons, including neurite intersections, branch points and total length [1]. ZEB85 (100 nM; 21 days) fully rescues the reduced dendritic complexity of BDNF-deficient primary mouse hippocampal PV+ interneurons and increases the dendritic complexity of BDNF-sufficient PV+ interneurons [1]. ZEB85 (10 nM; 24 h) shifts the dendritic spine type distribution of mature primary mouse hippocampal neurons toward more mushroom-type and fewer thin-type spines [1]. ZEB85 (100 nM; 21 h) completely reverses Aβ 1-42 -induced dendritic spine loss and altered spine type distribution in mature primary mouse hippocampal neurons [1]. ZEB85 (100 nM; 15 min-2 h) activates the downstream TrkB signaling pathway in mature primary mouse hippocampal neurons and upregulates the expression of cFOS and pERK, whereas 10 nM ZEB85 fails to significantly activate this signaling pathway [1]. ZEB85 (100 nM; 24 h) increases the amplitude of spontaneous calcium transients, a marker of neuronal activity, in mature primary mouse hippocampal neurons [1]. ZEB85 (100 nM; 5-6 days) rescues the LTP induction deficit in hippocampal organotypic cultures of BDNF het KO mouse brain slices, increases the success rate of LTP induction to 60%, and promotes stable and long-lasting synaptic potentiation [1]. ZEB85 (100 nM; 5-30 min) activates the canonical TrkB signaling pathway (phosphorylation of PLCγ, AKT, and ERK) in CHO-NFAT human TrkB reporter cells, with a magnitude and time course of effect similar to that of BDNF [2]. ZEB85 potently induces TrkB phosphorylation in γ-aminobutyric acidergic neurons derived from H9 human embryonic stem cells, with an EC 50 of 470 pM [2]. ZEB85 (50 ug/mL; 15-240 min) activates the phosphorylation of TrkB in cortical neurons of embryonic day 21 mice [2]. ZEB85 (50 ug/mL; 3 days) significantly enhances dendritic growth of retinal ganglion cells in cultured adult mouse retinal explants [2]. |
| Système d'expression |
Mammalian cells |
| Cible |
Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB, TRKB, NTRK2, Tropomyosin-related kinase B, BDNF/NT-3 growth factors receptor, Trk-B |
| Niveau d'endotoxines |
< 10 EU/mg |
| Pureté |
>95% purity as determined by SDS-PAGE. |
| Purification |
Protein A/G purified from cell culture supernatant. |
| Numéro d'accès |
Q16620 |
| Forme |
Liquid |
| Tampon de stockage |
0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
|
| Informations d'utilisation du produit |
| Application |
Dilution |
| ELISA |
1:2000-1:20000 |
| WB |
1:500-1:2000 |
|
|---|
| Stabilité et stockage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Noms alternatifs | ZEB85 |
|---|
| Contexte | BDNF/NT-3 growth factors receptor (TrkB/NTRK2) is a ~91 kDa protein. Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. 1. Yeo, GS. et al. (2004) Nature neuroscience 7, 1187-9. PMID: 154947312. Haniu, M. et al. (1995) Archives of biochemistry and biophysics 322, 256-64. PMID: 7574684 |
|---|
| Reférences |
1. Tacke C, et al. Actions of the TrkB Agonist Antibody ZEB85 in Regulating the Architecture and Synaptic Plasticity in Hippocampal Neurons. Front Mol Neurosci. 2022;15:945348. Published 2022 Jun 30. [HS892016]
2. Merkouris S, et al. Fully human agonist antibodies to TrkB using autocrine cell-based selection from a combinatorial antibody library. Proc Natl Acad Sci U S A. 2018;115(30):E7023-E7032. [HS892016] |
| Note |
For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control |
Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
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