Anti-SARS-CoV-2 RBD Reference Antibody (CV30, RUO) (VK565013)

Name: Anti-SARS-CoV-2 RBD Reference Antibody (CV30, RUO)
Brand: abinScience
SKU: VK565013
Price: 370 USD
Availability: InStock

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

VK565013

Description

Anti-SARS-CoV-2 RBD Antibody (CV30) (VK565013) is a research-grade recombinant antibody targeting Spike glycoprotein. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

Reactivité des espèces

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Applications

ELISA, Bioactivity: FCM, Functional assay, Research in vivo

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG1

ID de clone

CV30

Cible

Spike glycoprotein, Spike, S glycoprotein, RBD

Niveau d'endotoxines

< 10 EU/mg

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P0DTC2

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors.

1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651

2. Walls, AC. et al. (2020) Cell 181, 281-292.e6. PMID: 32155444

3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 33607086

4. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 34561887

5. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 32075877

6. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 32221306

7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715

8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763

9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294

10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

Detects Recombinant SARS-CoV-2 Spike protein receptor binding domain (Cat No.: VK565021) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-SARS-CoV-2 RBD Antibody (CV30).