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Anti-SARS-CoV-2 RBD Reference Antibody (VIR-7229, RUO) (VK565566)

Anti-SARS-CoV-2 RBD Reference Antibody (VIR-7229, RUO)
Anti-SARS-CoV-2 RBD Reference Antibody (VIR-7229, RUO)
Anti-SARS-CoV-2 RBD Reference Antibody (VIR-7229, RUO)
Anti-SARS-CoV-2 RBD Reference Antibody (VIR-7229, RUO)
Цена(USD):
Спецификация:
  • 100ug
  • 1mg
  • 5mg
  • 10mg
  • 25mg
  • 50mg
  • 100mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерVK565566
Реактивность видовSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
ПримененияELISA, Bioactivity: FCM, Functional assay, Research in vivo
Хозяинский видHuman
КлоональностьMonoclonal
ИзотипIgG1-kappa
ID клонаVIR-7229
Проверенные приложения WB
Цель Spike glycoprotein, Spike, S glycoprotein, RBD
Уровень эндотоксинов < 10 EU/mg
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P0DTC2
Форма Liquid
Буфер хранения 0.01M PBS pH 7.4.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияVIR-7229, VIR7229, VIR7229
Фон

Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors.

1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651
2. Walls, AC. et al. (2020) Cell 181, 281-292.e6. PMID: 32155444
3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 33607086
4. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 34561887
5. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 32075877
6. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 32221306
7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715
8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763
9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294
10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293
Ссылки 1. Rosen LE, et al. A potent pan-sarbecovirus neutralizing antibody resilient to epitope diversification. Cell. 2024 Dec 12;187(25):7196-7213.e26. [VK565566]
Примечание For research use only. Not suitable for clinical or therapeutic use.
Изображения
  • Anti-SARS-CoV-2 RBD Reference Antibody (VIR-7229, RUO)

    SDS-PAGE

    SDS-PAGE for Research Grade Anti-SARS-CoV-2 RBD Antibody (VIR-7229)

  • Anti-SARS-CoV-2 RBD Reference Antibody (VIR-7229, RUO)

    Western blot

    Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant SARS-CoV-2 RBD(Catalog No: VK565131)
    Lane 2: negative control

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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