Anti-SARS-CoV-2 S2 Protein Reference Antibody (CV3-25, RUO) (VK749026)

Name: Anti-SARS-CoV-2 S2 Protein Reference Antibody (CV3-25, RUO)
Brand: abinScience
SKU: VK749026
Price: 326 USD
Availability: InStock

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Precio(USD):

Especificación:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

VK749026

Descripción

Anti-SARS-CoV-2 S2 Protein (CV3-25) (VK749026) is a research-grade recombinant antibody targeting Spike protein S2'. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

Reactividad de especies

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Aplicaciones

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

IgG1

ID de clon

CV3-25

Sistema de expresión

Mammalian cells

Aplicaciones probadas

Objetivo

Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Spike protein S1, Spike protein S2, Spike protein S2', S, 2

Nivel de endotoxinas

< 10 EU/mg

Pureza

>95% purity as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P0DTC2

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Spike glycoprotein (Spike glycoprotein 2) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors.

1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651

2. Walls, AC. et al. (2020) Cell 181, 281-292.e6. PMID: 32155444

3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 33607086

4. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 34561887

5. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 32075877

6. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 32221306

7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715

8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763

9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294

10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293

Nota

For research use only. Not suitable for clinical or therapeutic use.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-SARS-CoV-2 S2 Protein Reference Antibody (CV3-25, RUO)
Western blot

Western blot analysis was performed using anti-SARS-CoV-2 S2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant SARS-CoV-2 S2/Spike S2 (ECD) (Catalog No: VK749032)
Lane 2: negative control
Bioactivity

Detects recombinant SARS-CoV-2 S2/Spike S2 (ECD) (Catalog No: VK749032) in indirect ELISA