Anti-SARS-CoV-2 S2 Protein Reference Antibody (CV3-25, RUO) (VK749026)

Name: Anti-SARS-CoV-2 S2 Protein Reference Antibody (CV3-25, RUO)
Brand: abinScience
SKU: VK749026
Price: 326 USD
Availability: InStock

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Спецификация:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

VK749026

Описание

Anti-SARS-CoV-2 S2 Protein (CV3-25) (VK749026) is a research-grade recombinant antibody targeting Spike protein S2'. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

Реактивность видов

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Применения

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

Хозяинский вид

Human

Клоональность

Monoclonal

Изотип

IgG1

ID клона

CV3-25

Система экспрессии

Mammalian cells

Проверенные приложения

Цель

Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Spike protein S1, Spike protein S2, Spike protein S2', S, 2

Уровень эндотоксинов

< 10 EU/mg

Чистота

>95% purity as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P0DTC2

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Spike glycoprotein (Spike glycoprotein 2) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors.

1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651

2. Walls, AC. et al. (2020) Cell 181, 281-292.e6. PMID: 32155444

3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 33607086

4. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 34561887

5. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 32075877

6. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 32221306

7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715

8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763

9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294

10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293

Примечание

For research use only. Not suitable for clinical or therapeutic use.

Изображения

SDS-PAGE

SDS-PAGE for Anti-SARS-CoV-2 S2 Protein Reference Antibody (CV3-25, RUO)
Western blot

Western blot analysis was performed using anti-SARS-CoV-2 S2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant SARS-CoV-2 S2/Spike S2 (ECD) (Catalog No: VK749032)
Lane 2: negative control
Bioactivity

Detects recombinant SARS-CoV-2 S2/Spike S2 (ECD) (Catalog No: VK749032) in indirect ELISA