Anti-Human CD45 Reference Antibody (Apamistamab, RUO) (HY195016)

Name: Anti-Human CD45 Reference Antibody (Apamistamab, RUO)
Brand: abinScience
SKU: HY195016
Price: 176 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HY195016
説明	Apamistamab (HY195016) is a research-grade recombinant antibody targeting CD45. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG1-kappa
発現システム	Mammalian cells
テスト済みアプリケーション	FCM
ターゲット	T200, Leukocyte common antigen, CD45, L-CA, Receptor-type tyrosine-protein phosphatase C, PTPRC
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P08575
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景	Receptor-type tyrosine-protein phosphatase C (CD45/PTPRC) is a ~147 kDa protein. Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. 1. Dunlock, VE. et al. (2022) Cell reports 39, 111006. PMID: 35767951
注意事項	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

イメージ

Bioactivity

Detects Human CD45/PTPRC in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD45 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD45 monoclonal antibody (Catalog HY195016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Research Grade Apamistamab