Anti-Human IL-1 alpha/IL-1F1 Reference Antibody (Bermekimab, RUO) (HF802016)

Name: Anti-Human IL-1 alpha/IL-1F1 Reference Antibody (Bermekimab, RUO)
Brand: abinScience
SKU: HF802016
Price: 176 USD
Availability: InStock

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100ug
1mg
5mg
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Vista general

Número de catálogo	HF802016
Descripción	Research Grade Bermekimab (HF802016) is a humanized monoclonal antibody detecting IL1F1 in ELISA, Bioactivity: FACS, Functional assay, Research in vivo. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications.
Reactividad de especies	Human
Aplicaciones	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Especie huésped	Human
Clonalidad	Monoclonal
Isotipo	IgG1-kappa
Sistema de expresión	Mammalian cells
Especies	Human
Aplicaciones probadas	FCM
Objetivo	IL1F1, Interleukin-1 alpha, IL1A, Hematopoietin-1, IL-1 alpha
Nivel de endotoxinas	< 10 EU/mg
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P01583
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	MABp1, Xilonix, CAS: 1401965-15-8, CA-18C3, CV-18C3, RA-18C3, T2-18C3
Fondo	Interleukin-1 alpha (IL1F1,Interleukin-1 alpha,IL1A,Hematopoietin-1,IL-1 alpha) is a ~30 kDa protein. Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. 1. Cohen, I. et al. (2015) Scientific reports 5, 14756. PMID: 26439902 2. Brikos, C. et al. (2007) Molecular & cellular proteomics : MCP 6, 1551-9. PMID: 17507369 3. Mosley, B. et al. (1987) The Journal of biological chemistry 262, 2941-4. PMID: 2950091 4. Wu, T. et al. (2004) Cytokine 25, 11-20. PMID: 14687581 5. Spiekstra, SW. et al. (2005) Experimental dermatology 14, 109-16. PMID: 15679580
Nota	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Imágenes

SEC-HPLC

SEC-HPLC detection for Research Grade Bermekimab.
Bioactivity

Detects IL1A/IL1F1 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Bermekimab.
Flow cytometry

Flow-cytometry using PE anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL1A monoclonal antibody (Catalog HF802016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human IL1A monoclonal antibody (Catalog HF802016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A monoclonal antibody (Catalog HF802016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.