Anti-Human Glypican 3 Reference Antibody (Codrituzumab, RUO) (HW310016)

Name: Anti-Human Glypican 3 Reference Antibody (Codrituzumab, RUO)
Brand: abinScience
SKU: HW310016
Price: 176 USD
Availability: InStock

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100ug
1mg
5mg
10mg
25mg
50mg
100mg

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Referencias
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SDS

Vista general

Número de catálogo	HW310016
Descripción	Codrituzumab (HW310016) is a research-grade recombinant antibody targeting GPC3. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Reactividad de especies	Human
Aplicaciones	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Especie huésped	Human
Clonalidad	Monoclonal
Isotipo	IgG1-kappa
Sistema de expresión	Mammalian cells
Aplicaciones probadas	WB, FCM
Objetivo	GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5
Nivel de endotoxinas	< 10 EU/mg
Pureza	>95% purity as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P51654
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	GC33, RG7686, RO5137382, 1365267-33-9
Fondo	Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. 1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063 2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623 3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449 4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790
Nota	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Imágenes

SEC-HPLC

SEC-HPLC detection for Research Grade Codrituzumab.
Bioactivity

Detects GPC3 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 monoclonal antibody (Catalog HW310016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Research Grade Codrituzumab.
Western blot

Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human GPC3 (Catalog No: HW310011), 50ng
Lane 2: recombinant human GPC3 (Catalog No: HW310011), 30ng