Anti-Human Glypican 3 Reference Antibody (Codrituzumab, RUO) (HW310016)

Name: Anti-Human Glypican 3 Reference Antibody (Codrituzumab, RUO)
Brand: abinScience
SKU: HW310016
Price: 176 USD
Availability: InStock

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100ug
1mg
5mg
10mg
25mg
50mg
100mg

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Изображения
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Даташит
SDS

Обзор

Каталожный номер	HW310016
Описание	Codrituzumab (HW310016) is a research-grade recombinant antibody targeting GPC3. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Реактивность видов	Human
Применения	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG1-kappa
Система экспрессии	Mammalian cells
Проверенные приложения	WB, FCM
Цель	GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5
Уровень эндотоксинов	< 10 EU/mg
Чистота	>95% purity as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P51654
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	GC33, RG7686, RO5137382, 1365267-33-9
Фон	Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. 1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063 2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623 3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449 4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790
Примечание	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Изображения

SEC-HPLC

SEC-HPLC detection for Research Grade Codrituzumab.
Bioactivity

Detects GPC3 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human GPC3 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 monoclonal antibody (Catalog HW310016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Research Grade Codrituzumab.
Western blot

Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human GPC3 (Catalog No: HW310011), 50ng
Lane 2: recombinant human GPC3 (Catalog No: HW310011), 30ng