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| カタログ番号 | HF610026 |
|---|---|
| 説明 |
Denecimig (HF610026) is a research-grade recombinant antibody targeting Stuart factor. Produced in mammalian cells with native-like glycosylation.
Highlights
|
| 種反応性 | Human |
| アプリケーション | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 宿主種 | Human |
| クローン性 | Monoclonal |
| アイソタイプ | IgG4-kappa |
| 発現システム | Mammalian cells |
| ターゲット | Stuart factor, Stuart-Prower factor, Coagulation factor X, F10, Plasma thromboplastin component, F9, Christmas factor, Coagulation factor IX, PTC |
| エンドトキシンレベル | < 10 EU/mg |
| 純度 | >95% purity as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P00742, P00740 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | 2488745-86-2 |
| 背景 | Coagulation factor X (F10/Factor X & F9) is a ~54 kDa protein. Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner. Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells. Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner. Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues. 1. Manithody, C. et al. (2012) Biochemistry 51, 2551-7. PMID: 22409427 2. Cao, Q. et al. (2025) Nature 639, 816-824. PMID: 39880037 3. Bukowska, A. et al. (2013) European journal of pharmacology 718, 114-23. PMID: 24041930 4. Shinozawa, E. et al. (2018) Frontiers in pharmacology 9, 1431. PMID: 30568593 5. D'Alessandro, E. et al. (2021) Cells 10. PMID: 34831181 6. Borensztajn, K. et al. (2008) The American journal of pathology 172, 309-20. PMID: 18202198 7. Shrivastava, G. et al. (2022) ImmunoHorizons 6, 373-383. PMID: 35738824 8. Senden, NH. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 161, 4318-24. PMID: 9780208 |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG4 (S228P) Recombinant Isotype Control Antibody (SAA0184) (HP298107) |
Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human F9 monoclonal antibody (Catalog HF610026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analyzed on a NovoCyte Flow Cytometer.
A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human F10 monoclonal antibody (Catalog HF610026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analyzed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human Coagulation Factor IX&Coagulation Factor X Reference Antibody (Denecimig, RUO)
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