Anti-Human PD-L1 Reference Antibody (Durvalumab, RUO) (HV974026)

Name: Anti-Human PD-L1 Reference Antibody (Durvalumab, RUO)
Brand: abinScience
SKU: HV974026
Price: 176 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HV974026
説明	Durvalumab (HV974026) is a research-grade recombinant antibody targeting CD1. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns. Resources ●Flow Cytometry Protocol for PD-L1 (CD274) Detection — Validated protocol on protocols.io
種反応性	Human
アプリケーション	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1-kappa
テスト済みアプリケーション	WB, FCM
ターゲット	B7 homolog 1, B7-H1, B7H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1
エンドトキシンレベル	< 10 EU/mg
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	Q9NZQ7
形態	Liquid
保存バッファー	0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	MEDI4736, 1428935-60-7
背景	Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~33 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi). _x005F_x000D_ _x005F_x000D_ 1. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 _x005F_x000D_ _x005F_x000D_ 2. Mezzadra, R. et al. (2017) Nature 549, 106-110. PMID: 28813410 _x005F_x000D_ _x005F_x000D_ 3. Burr, ML. et al. (2017) Nature 549, 101-105. PMID: 28813417 _x005F_x000D_ _x005F_x000D_ 4. Huang, X. et al. (2019) Cancer immunology research 7, 1580-1590. PMID: 31399419 _x005F_x000D_ _x005F_x000D_ 5. Kennedy, A. et al. (2023) The EMBO journal 42, e111556. PMID: 36727298 _x005F_x000D_ _x005F_x000D_ 6. Dong, H. et al. (1999) Nature medicine 5, 1365-9. PMID: 10581077 _x005F_x000D_ _x005F_x000D_ 7. Hou, J. et al. (2020) Nature cell biology 22, 1264-1275. PMID: 32929201 _x005F_x000D_
注意事項	For research use only. Not suitable for clinical or therapeutic use.

イメージ

SEC-HPLC

SEC-HPLC detection for Research Grade Durvalumab.
Bioactivity

Detects Human CD274/PD-L1/B7-H1 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog HV974036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Research Grade Durvalumab
Flow CytoMetry

Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD274 monoclonal antibody (Catalog: HV974026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using APC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD274 monoclonal antibody (Catalog: HV974026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD5 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD5 (Catalog No: HY046011), 50ng
Lane 2: recombinant human CD5 (Catalog No: HY046011), 30ng
Lane 3: recombinant human CD5 (Catalog No: HY046011), 20ng
Lane 4: recombinant human CD5 (Catalog No: HY046011), 10ng