Anti-Human IFN-gamma Reference Antibody (Emapalumab, RUO) (HF813016)

Name: Anti-Human IFN-gamma Reference Antibody (Emapalumab, RUO)
Brand: abinScience
SKU: HF813016
Price: 176 USD
Availability: InStock

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100ug
1mg
5mg
10mg
25mg
50mg
100mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HF813016
説明	Emapalumab (HF813016) is a research-grade recombinant antibody targeting Interferon gamma. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1-lambda
発現システム	Mammalian cells
テスト済みアプリケーション	WB
ターゲット	Interferon gamma, IFN-gamma, Immune interferon, IFNG
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P01579
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	NI-0501, emapalumab-lzsg, 1709815-23-5
背景	Interferon gamma (IFNG/IFN-gamma) is a ~19 kDa protein. Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. 1. El Bougrini, J. et al. (2006) Cellular and molecular biology (Noisy-le-Grand, France) 52, 9-15. PMID: 16914093 2. Hisamatsu, H. et al. (1996) The Journal of experimental medicine 183, 1807-16. PMID: 8666937 3. Greenlund, AC. et al. (1993) The Journal of biological chemistry 268, 18103-10. PMID: 8349687 4. Akiyama, K. et al. (1994) FEBS letters 343, 85-8. PMID: 8163024 5. Fabunmi, RP. et al. (2001) Journal of cell science 114, 29-36. PMID: 11112687 6. Lah, TT. et al. (1995) FEBS letters 363, 85-9. PMID: 7729559
注意事項	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1, lambda Isotype Control antibody (HyHEL-10) (HP248107)

イメージ

SDS-PAGE

SDS-PAGE for Research Grade Emapalumab.
Western blot

Western blot analysis was performed using anti-IFNG/IFN-gamma monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IFNG/IFN-gamma (Catalog No: HF813012), 50ng
Lane 2: recombinant human IFNG/IFN-gamma (Catalog No: HF813012), 30ng
Lane 3: recombinant human IFNG/IFN-gamma (Catalog No: HF813012), 20ng