| Numéro de catalogue | VK565336 |
|---|
| Reactivité des espèces | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) |
|---|
| Applications | ELISA, Functional assay, Research in vivo |
|---|
| Clonalité | Monoclonal |
|---|
| Isotype | DARpin |
|---|
| Activité biologique |
BA 1 (0.1 uM) significantly increases FAK tyrosine phosphorylation in BRS-3 transfected NCI-H1299 cells [1]. |
| Nature |
Recombinant |
| Cible |
Spike glycoprotein, Spike, S glycoprotein, RBD |
| Niveau d'endotoxines |
< 10 EU/mg |
| Pureté |
>95% as determined by SDS-PAGE. |
| Purification |
Purified by Nickel column. |
| Numéro d'accès |
P0DTC2 |
| Forme |
Lyophilized |
| Tampon de stockage |
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
|
| Expédition |
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stabilité et stockage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Noms alternatifs | MP0420, D614Gvariant, B.1.351/Beta, P.1/Gamma, B.1.617.2/Delta, AY.2/DeltaPlus, AY.4.2/DeltaPlus, Lambda(C.37), Mu(B.1.621), Omicron(B.1.1.529, BA.1), Omicron(B.1.1.529, BA.2) |
|---|
| Contexte | Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors. 3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 336070864. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 345618875. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 320758776. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 322213068. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 367797639. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 3308229410. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293 |
|---|
| Reférences |
1. Terry W Moody, et al. ML-18 is a non-peptide bombesin receptor subtype-3 antagonist which inhibits lung cancer growth. Peptides. 2015 Feb;64:55-61. [VK565336] |
| Note |
For research use only. Not suitable for clinical or therapeutic use. |
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