Anti-Human IGF-I R/IGF1R Reference Antibody (Ganitumab, RUO) (HY353026)

Name: Anti-Human IGF-I R/IGF1R Reference Antibody (Ganitumab, RUO)
Brand: abinScience
SKU: HY353026
Price: 176 USD
Availability: InStock

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100ug
1mg
5mg
10mg
25mg
50mg
100mg

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo	HY353026
Descripción	Ganitumab (HY353026) is a research-grade recombinant antibody targeting CD221. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Reactividad de especies	Human
Aplicaciones	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Especie huésped	Human
Clonalidad	Monoclonal
Isotipo	IgG1-kappa
Sistema de expresión	Mammalian cells
Aplicaciones probadas	FCM
Objetivo	CD221, Insulin-like growth factor 1 receptor, IGF1R, Insulin-like growth factor I receptor, IGF-I receptor
Nivel de endotoxinas	< 10 EU/mg
Pureza	>95% purity as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P08069
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	AMG479, 905703-97-1
Fondo	Insulin-like growth factor 1 receptor (CD221/IGF1R) is a ~154 kDa protein. Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins.
Nota	For research use only. Not suitable for clinical or therapeutic use.

Imágenes

Bioactivity

SEC-HPLC detection for Research Grade Ganitumab.
Flow Cytometry

Flow-cytometry using anti-human IGFIR antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGFIR monoclonal antibody (Catalog HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human IGFIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human IGFIR monoclonal antibody (Catalog HY353026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human IGF-I R/IGF1R Reference Antibody (Ganitumab, RUO)
Flow CytoMetry

Flow-cytometry using APC anti-human CD221 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD221 monoclonal antibody (Catalog: HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD221 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD221 monoclonal antibody (Catalog: HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.