Anti-Human IGF-I R/IGF1R Reference Antibody (Ganitumab, RUO) (HY353026)

Name: Anti-Human IGF-I R/IGF1R Reference Antibody (Ganitumab, RUO)
Brand: abinScience
SKU: HY353026
Price: 176 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HY353026
説明	Ganitumab (HY353026) is a research-grade recombinant antibody targeting CD221. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1-kappa
発現システム	Mammalian cells
テスト済みアプリケーション	FCM
ターゲット	CD221, Insulin-like growth factor 1 receptor, IGF1R, Insulin-like growth factor I receptor, IGF-I receptor
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P08069
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	AMG479, 905703-97-1
背景	Insulin-like growth factor 1 receptor (CD221/IGF1R) is a ~154 kDa protein. Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins.
注意事項	For research use only. Not suitable for clinical or therapeutic use.

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Bioactivity

SEC-HPLC detection for Research Grade Ganitumab.
Flow Cytometry

Flow-cytometry using anti-human IGFIR antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGFIR monoclonal antibody (Catalog HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human IGFIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human IGFIR monoclonal antibody (Catalog HY353026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human IGF-I R/IGF1R Reference Antibody (Ganitumab, RUO)
Flow CytoMetry

Flow-cytometry using APC anti-human CD221 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD221 monoclonal antibody (Catalog: HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD221 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD221 monoclonal antibody (Catalog: HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.