Anti-Human MICA/B Reference Antibody (IPH4301, RUO) (HS894016)

Name: Anti-Human MICA/B Reference Antibody (IPH4301, RUO)
Brand: abinScience
SKU: HS894016
Price: 176 USD
Availability: InStock

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100ug
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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HS894016

種反応性

Human

アプリケーション

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

宿主種

Human

クローン性

Monoclonal

アイソタイプ

IgG1-kappa

クローンID

IPH4301

生物学的活性

IPH4301 induces death of tumor cells expressing MICA/B in vitro via antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis [1]. IPH4301 blocks the binding of MICA/B to NKG2D, and restores the expression and function of NKG2D on primary human NK cells and T cells [1]. IPH4301 reverses the immunosuppressive effect induced by M2 macrophages and restores the ADCC activity of autologous NK cells against MICA-expressing tumor cell lines [1]. IPH4301-derived PEG-G5.NH2-FITC-DOTA(Gd)-Monalizumab/IPH4301 nanoprobe can efficiently bind to NK-92MI cells. Its binding efficiency and in vitro MRI signal increase with elevated concentrations; besides, it upregulates IFN-γ secretion of NK-92MI cells, enhances the cytotoxicity against MDA-MB-231 triple-negative breast cancer cells and induces tumor cell apoptosis [2].

発現システム

Mammalian cells

ターゲット

MICA, MIC-A, MHC class I polypeptide-related sequence A, PERB11.1, MHC class I polypeptide-related sequence B, MIC-B, MICB, PERB11.2

エンドトキシンレベル

< 10 EU/mg

純度

>95% purity as determined by SDS-PAGE.

精製

Protein A/G purified from cell culture supernatant.

アクセッション番号

Q29983, Q29980

形態

Liquid

保存バッファー

0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:2000-1:20000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

別名

IPH4301

背景

MHC class I polypeptide-related sequence A (MICA/MICB) is a ~42 kDa protein. Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.

1. Steinle, A. et al. (2001) Immunogenetics 53, 279-87. PMID: 11491531

2. Sutherland, CL. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 671-9. PMID: 11777960

3. Bauer, S. et al. (1999) Science (New York, N.Y.) 285, 727-9. PMID: 10426993

参考文献

1. Morel A, et al. Abstract 1491: IPH4301, an antibody targeting MICA and MICB exhibits potent cytotoxic activity and immunomodulatory properties for the treatment of cancer. Cancer Res. 2016 Jul;76(14 Suppl):1491. [HS894016]
2. Wang Z, et al. Preliminary study on PEG-G5.NH2-FITC-DOTA(Gd)-Monalizumab/IPH4301 probe. Biotechnol Bull. 2023;10. [HS894016]

注意事項

For research use only. Not suitable for clinical or therapeutic use.

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