Anti-Human CD276/B7-H3 Reference Antibody (Mirzotamab, RUO) (HV612036)

Name: Anti-Human CD276/B7-H3 Reference Antibody (Mirzotamab, RUO)
Brand: abinScience
SKU: HV612036
Price: 176 USD
Availability: InStock

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100ug
1mg
5mg
10mg
25mg
50mg
100mg

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Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo	HV612036
Descripción	Mirzotamab (HV612036) is a research-grade recombinant antibody targeting CD276. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Reactividad de especies	Human
Aplicaciones	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Especie huésped	Human
Clonalidad	Monoclonal
Isotipo	IgG1-kappa
Aplicaciones probadas	WB, FCM
Objetivo	4Ig-B7-H3, B7 homolog 3, B7-H3, B7H3, CD276, CD276 antigen, Costimulatory molecule
Nivel de endotoxinas	< 10 EU/mg
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	Q5ZPR3
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	ABBV-155, 2229859-11-2
Fondo	CD276 antigen (B7-H3) is a ~57 kDa protein. May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. CD276 is the therapeutic target of omburtamab (clinical).
Nota	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Imágenes

SEC-HPLC

SEC-HPLC detection for Research Grade Mirzotamab.
Bioactivity

Detects CD276/B7-H3 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Mirzotamab.
Flow CytoMetry

Flow-cytometry using APC anti-human CD276 antibody. PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD276 monoclonal antibody (Catalog: HV612036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD276 antibody. PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD276 monoclonal antibody (Catalog: HV612036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD276 antibody. PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD276 monoclonal antibody (Catalog: HV612036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD276 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD276 (Catalog No: HV612011)