Anti-Human CD28 Reference Antibody (Pegrizeprument, RUO) (HY422056)

Name: Anti-Human CD28 Reference Antibody (Pegrizeprument, RUO)
Brand: abinScience
SKU: HY422056
Price: 370 USD
Availability: InStock

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Spécification :

100ug
1mg
5mg
10mg
25mg
50mg
100mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY422056

Description

Pegrizeprument (HY422056) is a research-grade recombinant antibody targeting CD28. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

Reactivité des espèces

Human

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

Espèce hôte

Chimeric

Clonalité

Monoclonal

Isotype

Immunoglobulin Fab VH-G1CH1h_L-kappa

Système d'expression

Mammalian cells

Applications testées

Cible

CD28, TP44, T-cell-specific surface glycoprotein CD28

Niveau d'endotoxines

< 10 EU/mg

Pureté

>95% purity as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P10747

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Noms alternatifs

2829316-24-5

Contexte

T-cell-specific surface glycoprotein CD28 is a ~25 kDa protein. Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.

1. Gimmi, CD. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 6575-9. PMID: 1650475

2. Raab, M. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8891-5. PMID: 7568038

3. Skånland, SS. et al. (2014) The Biochemical journal 460, 399-410. PMID: 24665965

4. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291

5. Wu, H. et al. (2022) Structure (London, England : 1993) 30, 803-812.e5. PMID: 35397202

6. Kong, KF. et al. (2011) Nature immunology 12, 1105-12. PMID: 21964608

7. Blotta, MH. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3133-40. PMID: 8617933

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Pegrizeprument.
Western blot

Western blot analysis was performed using anti-CD28 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD28 (Catalog No: HY422011)
Lane 2: Negative control